Determination of apoptosis on fibronectin and collagen iv adhered 5RP7 cells [Fi·bronekti·n ve kolajen iv'e bagli 5RP7 hücreleri·nde apoptozun beli·rlenmesi·]

Abstract

Extracellular proteins such as fibronectin and collagen IV have a function in many cellular events such as cell growth, cell differentiation, tissue formation, cell adhesion, wound healing as well as have an important effects in the formation of cancer, the development of metastasis, and apoptosis. Extracellular proteins of fibronectin and collagen IV, depending on the type of tissue and cell have been shown to cause stimulation or inhibition of apoptotic mechanisms. In this study, using fibronectin or collagen IV protein-adhered cancer rat embryo fibroblast cells, cancer of the apoptotic mechanism in the embryonic period was investigated. Therefore, after the binding ratios fibronectin or collagen IV-adhered cells were calculated, the apoptotic indices were determined. It was identified that these cells by binding to fibronectin induced late apoptosis, but this effect was not observed on collagen IV adhered cells. During the mechanism of fibronectin-induced late apoptosis, caspase-3 and -9 were activated, but inhibition studies were shown that the mechanism of caspase-3 was more effective.