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dc.contributor.authorGaripcan, B
dc.contributor.authorSay, Rıdvan
dc.contributor.authorPatir, S
dc.contributor.authorArıca, Y
dc.contributor.authorDenizli, Adil
dc.date.accessioned2019-10-20T09:30:48Z
dc.date.available2019-10-20T09:30:48Z
dc.date.issued2002
dc.identifier.issn0920-5063
dc.identifier.urihttps://dx.doi.org/10.1163/15685620260178409
dc.identifier.urihttps://hdl.handle.net/11421/17470
dc.descriptionWOS: 000177648400005en_US
dc.descriptionPubMed ID: 12182559en_US
dc.description.abstractDifferent adsorbents have been reported in the literature for protein purification. The authors have developed a novel and new approach to obtain high protein adsorption capacity utilizing a 2-methacrylamidoalanine-containing membrane. Amino acid ligand 2-methacrylamidoalanine (MAAL) monomer was synthesized using methacryloyl chloride and alanine. Poly(2-hydroxyethylmethacrylate-co-2-methacrylamidoalanine) [p(HEMA-co-MAAL)] membranes were then prepared by UV-initiated photopolymerization of HEMA and MAAL in the presence of an initiator (azobisisobutyronitrile, AIBN). The synthesized MAAL monomer was characterized by NMR. p(HEMA-co-MAAL) membranes were characterized by swelling studies, porosimeter, SEM, FTIR, and elemental analysis. These membranes have macropores in the size range of 5-10 mum. Cu(II) ions (25.9 mmol/m(2)) were chelated on these membranes. p(HEMA-co-MAAL) membranes were used to study the adsorption of lysozyme from aqueous media containing different amounts of lysozyme (0.1-3.0 mg/l) and at different pH values (4.0-8.0). The non-specific adsorption of lysozyme on the pHEMA membranes was negligible (0.9 mug/cm(2)). Incorporation of MAAL increased the lysozyme adsorption significantly up to 2.96 mg/cm(2). The lysozyme adsorption capacity of the Cu(II) incorporated membranes (9.98 mg/cm(2)) was greater than that of the p(HEMA-co-MAAL) membranes. More than 90% of the adsorbed lysozyme was desorbed in I h in the desorption medium containing 1.0 M NaCl and 0.025 M EDTA. The metal-chelate affinity membranes are suitable for repeated use for more than ten cycles without a noticeable loss of capacity.en_US
dc.language.isoengen_US
dc.publisherVsp BVen_US
dc.relation.isversionof10.1163/15685620260178409en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLysozyme Adsorptionen_US
dc.subjectAmino Acid Membranesen_US
dc.subjectPoly(Hema)en_US
dc.subjectMetal Chelatesen_US
dc.titlePoly(hydroxyethyl methacrylate-co-methacrylamidoalanine) membranes and their utilization as metal-chelate affinity adsorbents for lysozyme adsorptionen_US
dc.typearticleen_US
dc.relation.journalJournal of Biomaterials Science-Polymer Editionen_US
dc.contributor.departmentAnadolu Üniversitesi, Fen Fakültesi, Fizik Bölümüen_US
dc.identifier.volume13en_US
dc.identifier.issue5en_US
dc.identifier.startpage563en_US
dc.identifier.endpage577en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorSay, Rıdvan


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