A study of calcium release from rat liver microsomes by thapsigargicin induction [Tapsigargisin indüksiyonu ile si{dotless}çan karaciger mikrozomlari{dotless}ndan kalsiyum sali{dotless}ni{dotless}mi{dotless} üzerine bir çali{dotless}şma]

Abstract

The primary aim of the study was to test the influences of thapsigargicin in releasing Ca 2+ from the intracellular Ca 2+ stores and comparing it with the effects of thapsigargin. In this study, the effect of tumour promoter thapsigargicin on intracellular Ca 2+ has been described. Therefore, rat liver endoplasmic reticulum subcellular fractions (microsomes) were employed and Ca 2+ movements measured by spectrofluorimeter. Fluo-3 studies were used to follow Ca 2+ release in experiments with microsomes. When results are evaluated, it has been concluded that like thapsigargin, thapsigargicin has the ability of discharging the intracellular Ca 2+ stores, increasing the intracellular free Ca 2+ concentration ([Ca 2+]i) and being a potent and specific inhibitor of the ER Ca 2+-ATPase.