Changes on cell surface and microfilament distribution during myoblast differentiation and transformation in vitro

Abstract

A myoblast cells that normally differentiated into myotubes are diverted into cell proliferetion following the activity of an oncogene. In this study, we have examined the CO25 myoblast cell line which are transfected human N-ras oncogene at different stage in the biological processes as differentiation and transformation for morphological and cytoskeletal changes. The phase contrast, fluorescence and scanning electron microscope were used for organization of the cell shape, details of the surface and microfilament organization. Certain differentiation were determined on the surface of cells, shape of the cells and also cytoplasmic cytoskeletal distribution between differentiated and transformed cells in different culture media.