Bacteriocin production by bean root bacteria

Abstract

Bacteriocin production of native isolates of Rhizobium was investigation. Six isolates of bacteriocin producing Rhizobium bacteria were isolated from bean root nodules in Turkey. Isolate Y39 characterized in this study was one of the most effective isolates. The possible bacteriocin from produced by Rhizobium spp. isolate Y39 was inhibitory to a broad range of indicator microorganisms, including Micrococcus sp., Bacillus isolates CB and CB1, Actinomycetes isolates A1 and A5, Azotobacter spp. isolates C12 and C35, Pseudomonas phaseolicola, Xanthomonas phaseolicola, Fusarium culmorum, F.moniliforme, F.solani, F.oxysporum, Cladosporium herbarum and Rhizoctonia solani. Bacteriocin was stable at 60 degrees C but the activity was lost when the temperature used at bacteriocins. It was resistant to RNAase and lysozyme but sensitive to proteinase K and trypsin. Some of properties of the compound showed suggest a proteinaceous nature.