Antiangiogenic and antiproliferative effects of halotolerant Penicillium chrysogenum var. chrysogenum on the human umbilical vein endothelial cells (HUVECs)

Abstract

Inhibitors of angiogenesis have been currently developed which offer the hope of new treatment options such as diabetic retinopathy, psoriasis, rheumatoid arthritis and cancer. In this study was to investigate the antiproliferative and antiangiogenic effects of ethylacetate extract of Penicillium chrysogenum var. chrysogenum on the HUVECs. Real-time cell analysis system was used to analyze antiproliferative and antiangiogenic effects according to change of cell index values. In our study different concentrations of extract for cell prolifetation analysis were investigated. Antiangiogenic effect was evaluated according to invasion and migration activity. Antiproliferative effects of extract were increased on HUVECs according to concentration and time depending. IC50 concentration was analyzed as 78.97 mu g/mL on 72h. In the invasion and migration assays, 50 mu g/mL extract concentration significantly suppressed angiogenic responses. This finding suggests that extract be potentially more pharmacologically active. According to these results natural antioxidants gained from fungi are very essential resources in terms of biotechnology.