The determination of prazosin hydrochloride in pharmaceutical preparations by flow injection analysis using UV-detection

Abstract

A flow Injection Analysis(FIA) of prazosin hydrochloride (PRZ) using UV detection is described. Methanolic HCl(0.2 mol.l-1) was used as the solvent. A flow rate of 1 ml.min-1 was pumped and the active material was detected at 245 nm. The calibration equation was linear in the range of 1×10-6-5×10-6 mol.l-1. Limit of detection (LOD) and limit of quantitation(LOQ) were caculated to be 1.3×10-7 and 3.2×10-7 mol.l-1, respectively. The proposed method was applied to the determination of PRZ in pharmaceutical preparations. the results were compared with those obtained from UV-spectro-photometry.